DNA

Part:BBa_K4761050

Designed by: Leidun Xu   Group: iGEM23_HiZJU-China   (2023-09-29)


Transposon Left End

Transposon left end designed for gene knock-in.

The left flanking sequence of the transposon on the pDonor plasmid, together with the gene fragment to be transferred (Cargo) and the Transposon Right End (BBa_K4761051), forms a complete Mini-transposon [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[1]Klompe, S.E., Vo, P.L.H., Halpin-Healy, T.S. et al. Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration. Nature 571, 219–225 (2019). https://doi.org/10.1038/s41586-019-1323-z

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